rectangular glass capillaries 5010  (VitroCom Inc)

Journal: Microfluidics and Nanofluidics

Article Title: A practical microfluidic pump enabled by acoustofluidics and 3D printing

doi: 10.1007/s10404-020-02411-w

Figure Lengend Snippet: Acoustic streaming profiles inside the rectangular glass capillary are visualized using streak images. At lower frequencies, more specifically at a 61.7 kHz, b 62.5 kHz, c 63.7 kHz and d 67.3 kHz, acoustic streaming flows results in more directional fluid displacement within the capillary. At higher frequencies of e 220 kHz and f 187 kHz, acoustic streaming-induced flows yield rotating vortices at different planes. In f —i and f —ii, two adjacent vortices are shown side by side because they could not fit in the same frame due their larger spatial distribution in the capillary. Yellow arrows are used to indicate the streaming directions. g The streaming profiles are recorded from the region of interest (ROI) shown with a red rectangle (color figure online)

Article Snippet: Schematic depiction of the a assembled and b side views are shown. c Photograph of an assembled device For the device assembly, two adaptors are positioned to have their capillary ports facing to each other, and two rectangular glass capillaries (5010, Vitrocom, NJ, USA) with 50 mm length, 0.1 mm height, 1 mm width and 0.07 mm wall thickness are inserted to each adaptor as shown in Fig. b.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: The Temperature Gradient-Forming Device, an Accessory Unit for Normal Light Microscopes To Study the Biology of Hyperthermophilic Microorganisms

doi: 10.1128/AEM.00984-14

Figure Lengend Snippet: The temperature gradient-forming device, including the control unit. This low-budget custom-made device consists of a stainless steel plate (A) located on a circuit board (B). Two independently working heating elements (C) located at the left and right ends of the steel plate allow heating up to 110°C. A rectangular glass capillary containing the cells to be analyzed is placed into the rectangular groove milled into the steel plate; the five small holes (the middle one is indicated by the arrow) for observation of cells are 5 mm apart each. On the back side of the circuit board, five temperature sensors are located close to the observation holes. The control unit (D) shows the actual temperatures at the left- and rightmost observation holes (here 74 and 110°C). The temperatures of the three middle observation holes (here 88°C for the middle hole indicated by the arrow) can be accessed using the black switch (E). The placement of the circuit board onto the xy-stage of any conventional upright light microscope converts it into a thermomicroscope.

Article Snippet: Briefly, cells in the logarithmic growth phase were transferred to rectangular glass capillaries (order no. 5010-100; VitroCom, Mountain Lakes, NJ, USA), which were closed by cyanoacrylate glue (Pattex Uni-Rapide [Henkel AG, Düsseldorf, Germany] or UHU Super Power [UHU Gmbh, Bühl, Germany]), and motility was analyzed using an Olympus BX50 microscope.

Techniques: Control, Light Microscopy